As very small amount of blood sample’s collected on filter paper, it requires intense diagnosis to achieve accurate result with specificity and sensitivity (Pieter et al. Samples, such as finger-prick blood and mosquito blood meal, are easily, quickly and safely collected onto filter paper, stored at room temperature and easily transported to the testing laboratory. The new method by using dried blood spots (DBS) can solve all the problems (Mastronardi et al. When performing epidemiological or diagnostic surveys in remote area, it is difficult for collection, storage and transportation of samples (Wheat 2001). Surveillance and diagnosis of malaria is difficult, in remote inaccessible area where facilities for storing and processing of samples do not exist. The purpose of this paper is to update the knowledge and find a method to extract DNA from DBS which will be specific, rapid, cost-effective, less time consuming and feasible for epidemiological survey in remote area. Efforts have been taken to identify the methods which are cost-effective and take less time to extract DNA from dried blood spots (DBS) and whole mosquitoes. The main DNA isolation methods are Chelex-100, Tris-EDTA (TE) buffer Methanol based DNA extraction and Phosphate buffer saline (PBS) using Lysis buffer and Phenol–Chloroform method. In this review we are focused upon four simple methods of DNA isolation from the field collected blood and mosquito abdomen blood meal spotted on Whatman No. In field particularly in remote area where facilities for storing and processing of samples does not exist, there surveillance and diagnosis of malaria is very difficult.

When performing malaria epidemiological surveys in remote area, it is difficult to collect blood samples and transport it. DNA extraction from filter paper by using different methods was compiled through a thorough review of many research articles published in various journals.

Te buffer recipe